About this manual. This manual describes the operation of the ÄKTA™FPLC™ system. System description, system maintenance and trouble-shooting are also. filter. Sample pump. Buffer select valve (V6). Injection valve (V1). Reverse . Manual. Flowpath. B1 or B2. BufferValveB. Pump A. Pump B. Common inlet. A2. A1. About this manual This manual describes the operation of ÄKTA purifier: Evaluating the UV response Calculate the UV response ratios in the following.

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Be sure not to proceed until the instructions are clearly understood and all stated conditions are met. Registration form posted to Service Administration.

ÄKTApurifier User Guide – GE Healthcare Life Sciences

The mains power switch must always be easy to access, and the purifjer plug must always be easy to disconnect. Each run in the scouting scheme will generate a separate result file which are all stored in a special scouting directory.

Settings in the System Control module and then Specials.

The flow cell itself does not have a recommended flow direction. Remove the column and the pH electrode optional. The flow cell should not be used with aka other pH electrode. Recycling This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately.

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Brief descriptions of the individual components are given in Section 8. The illustration to the right shows setting of the wavelength nm. BufferPrep is optimized for use in ion exchange chromatography method development, but can also be used for size exclusion chromatography and for affinity chromatography. Low eluent flow and noise as the piston moves 1 Replace or re-install the seal or membrane.


This is putifier base with only one pKa. There’s no available product features to display for this product variant. The first one is active as long as the pressure is below the set point.

For prep mode, two sizes of racks for tube diameter of 30 mm tubes, and ml bottles are available.

ÄKTApurifier – GE Healthcare Life Sciences

Make the appropriate selections in the dialog and then click OK to view the inserted item. Check if there is too low concentration of buffer components causing low buffering capacity. The length of each block is marked at the bottom of the graph. A pressure sensor is connected to pump module A left hand pair of pump heads.

Installation Test results evaluated. The sample loop is filled manually mannual a syringe and a fill port connected to port 3 in the injection valve. You can change this name and select a new directory by clicking Browse If not, purge the pump with a syringe as purifiet in 2. Looking for support assistance? The blocks represent the typical steps in a chromatographic run: When using the fraction collector 1 Check that the tubing from the flow restrictor is connected to the fraction collector.


It is important that the recommendations in these tables are followed. The sample loop must be completely filled with buffer before the sample can be loaded.

ÄKTApurifier User Guide – GE Healthcare Life Sciences |

Two techniques can be used for filling the sample loop; partial or complete filling. This will be a negative value for an acid, 0 for a base.

To set a useful pH range the pKa must be known. Change channel plate and distribution plate. Make sure that the upper pressure limit of the system is lower than the maximum allowed pressure of the Superloop. Manua, the open end in the waste container. When changing the filter, use a tool if necessary, to unscrew the filter body. These valves can be used to accomplish the following functions: