Petite H(1), Frei V, Huc A, Herbage D. Author information: (1)Coletica, Lyon, France. Cross-linking of collagen-based biomaterials increases their strength and. By DANIEL HERBAGE, JOSETTE BOUILLET and JEAN-CLAUDE Solubilization of collagen from bovine articular cartilage with pepsin. The formation of new bonds between the denatured collagen chains and the proteoglycans can explain the D. Herbage, A. Huc, D. Chabrand, M.C. Chapuy.

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Collagen-based biomaterials as 3D scaffold for cell cultures: Mouse fibroblasts in long-term culture within collagen three-dimensional scaffolds: Howard The Journal of biological chemistry DPPA-treated pericardium had a resistance to collagenase digestion similar to collageh of glutaraldehyde- or hydrazine-treated pericardium.

Cross-linking of dermal sheep collagen using a water-soluble carbodiimide. RosenthalGabriele J Koehler Anticancer research Daniel Herbage 1 AuthorId: In the 10 7 cells-seeded sponges, mineral deposition, observed with unseeded sponges, was significantly decreased 2- to 3-fold. Both supports were always present during the study and a partial decrease in size and weight was detected only with control sponges, both seeded and unseeded.

Collagen-based biomaterials in the form of sponges bovine type I collagen, both native and cross-linked by treatment with diphenylphosphorylazide, noted control and DPPA sponges respectively were tested as three-dimensional scaffolds to support chondrocyte proliferation with maintenance of the phenotype in order to form neocartilage.


Ham A Benghuzzi Biomedical sciences instrumentation BonassarCharles A. Have you forgotten your login? Similar decreases in free primary amino group content and increases in thermal stability were obtained for collagen films treated with DPPA, glutaraldehyde, or hydrazine.

Collagen – References

These in vitro results indicate that both collagen matrices can support the development of tissue engineered cartilage. BiomaterialsElsevier,22, pp. Files produced by the author s. Immunologically active biomaterials for cancer therapy.

Showing of 84 references. Vacanti Journal of cellular biochemistry.

Citations Publications citing this paper. Cross-linking of collagen-based biomaterials increases their strength and persistence in vivo. Colagen accumulated in the matrix, up to 1.

Use of diphenylphosphorylazide for cross-linking collagen-based biomaterials.

Ocllagen cartilage collagens types II and XI were deposited in the matrix throughout the culture and traces of type I collagen were noticed only in the culture medium after weeks and 4 weeks in the case of 10 6 and 10 7 cells-seeded sponges, respectively. In this report, we propose a simpler, faster way to prepare acyl azide groups and to cross-link collagen-based biomaterials, using diphenylphosphorylazide DPPA as a collaggen.

In order to validate and quantitate the extent of reaction, the degree of cross-linking was determined by the measure of the free primary amino group content of the samples. Treatment of native bovine pericardium with 0.

Wednesday, December 13, – 2: Methods for the treatment of collagenous tissues for bioprostheses. Aluminum Oxide Search for additional papers on this topic.


Use of diphenylphosphorylazide for cross-linking collagen-based biomaterials.

Cytological evaluation of capsular tissue surrounding TCPL implant in adult rats. ChevallayDr D. Gelatinase A activation is regulated by the organization of the polymerized actin cytoskeleton. Skip to search form Skip to main content. Collagen as matrix for neo-organ formation by gene-transfected fibroblasts. Anne-Marie Freyria 1 AuthorId: By clicking accept or continuing to use the site, you agree to the terms outlined in our Privacy PolicyTerms of Serviceand Dataset License.

Allogeneic Mesenchymal Stem Cells and Biomaterials: The Perfect Match for Cardiac Repair?

Cell proliferation was only noted in the 10 6 cells-seeded sponges 4-fold increase after 4 weeks of culture. Chevallay and Dr D. After determining the optimal conditions of cross-linking coolagen DPPA, we compared the efficiency of this protocol with that using hydrazine and with the classical glutaraldehyde treatment.

With the rapid development in tissue engineering, these different biomaterials have been used as three-dimensional scaffolds and cell transplant devices. These results were corroborated by resistance to bacterial collagenase digestion: AliDavid J Mooney Current topics in microbiology and immunology From This Paper Figures, tables, and topics from this paper. Mineral deposition Glycosaminoglycan deposition Articular cartilage repair Collagen-based biomaterial Collagen synthesis.